Transcriptomics

Dataset Information

76

RNAseq of human epithelial lung carcinoma cell line A549 exposed to ceria, silver or zinc ions, micron-sized particles or nanoparticles


ABSTRACT: The toxicity of silver and zinc oxide nanoparticles is hypothesised to be mediated by dissolved metal ions and cerium dioxide nanoparticles (CeO2 NPs) are hypothesised to induce toxicity specifically by oxidative stress dependant on their surface redox state. To test these hypotheses, RNAseq was applied to characterise the molecular responses of cells to metal nanoparticle and metal ion exposures. The human epithelial lung carcinoma cell line A549 was exposed to different CeO2 NPs with different surface charges, micron-sized and nano-sized silver particles and silver ions, micron-sized and nano-sized zinc oxide particles and zinc ions, or control conditions, for 1 hour, 6 hours and 24 hours. Concentrations were the lower of either EC20 or 128 micrograms/mL. Transcriptional responses were characterised by RNAseq transcriptomics using an Illumina HiSeq2500 .

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo Sapiens

SUBMITTER: Tim D Williams  

PROVIDER: E-MTAB-5734 | ArrayExpress | 2018-02-01

SECONDARY ACCESSION(S): ERP022839

REPOSITORIES: ArrayExpress, ENA

Dataset's files

Source:
Action DRS
E-MTAB-5734.idf.txt Idf
E-MTAB-5734.idf.txt_original Idf
E-MTAB-5734.sdrf.txt Txt
Items per page:
1 - 3 of 3

Similar Datasets

| E-MTAB-5734 | BioStudies
2018-06-12 | GSE89653 | GEO
2022-06-15 | GSE151954 | GEO
| S-EPMC7915389 | BioStudies
2012-01-05 | E-GEOD-33278 | ArrayExpress
| S-EPMC8193676 | BioStudies
| S-EPMC4237954 | BioStudies
| S-EPMC6139999 | BioStudies
2017-03-21 | GSE90457 | GEO
| S-EPMC4830720 | BioStudies