Transcriptomics

Dataset Information

316

Lotus japonicus response to Nod factor: wild type and nfre mutants


ABSTRACT: For RNA sequencing, 3 days seedlings were moved to agar plates supplemented with 1/4 B&D media and susceptible zone of 14 days-old plants was harvested after specific treatment with water (Mock treated) or M.loti Nod factor 10-8M (NF). The total RNA was isolated from the susceptible zone (15 mm root pieces) using Nucleo spin RNA plant (Macherey-Nagel). Total RNA (> 0.8 g) from two biological replicas per sample was used by GATC Biotech (Germany) to prepare random primed cDNA library and for sequencing with Illumina HiSeq: read length 1 x 50bp. Gifu- L. japonicus wild-type, 4820- nfre-1 allele of Nfre, 38534- nfre-2 allele of Nfre.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Lotus japonicus  

SUBMITTER: Simona Radutoiu   Eiichi Murakami  

PROVIDER: E-MTAB-5855 | ArrayExpress | 2018-06-27

SECONDARY ACCESSION(S): ERP023945

REPOSITORIES: ArrayExpress, ENA

Similar Datasets

| GSE73069 | GEO
2013-11-01 | E-MTAB-2000 | ArrayExpress
| PRJNA10751 | ENA
2014-02-13 | E-GEOD-44391 | ArrayExpress
2015-08-28 | E-GEOD-55592 | ArrayExpress
2013-08-10 | E-GEOD-49707 | ArrayExpress
2015-11-05 | PXD002540 | Pride
2012-02-22 | E-GEOD-31823 | ArrayExpress
2016-01-26 | PXD003378 | Pride
2016-07-26 | PXD003504 | Pride