Transcriptomics

Dataset Information

332

Single-cell RNA-seq of dermal fibroblasts stimulated with dsRNA (poly I:C) in a time course of 0, 2, 4 and 8 hours


ABSTRACT: Dermal fibroblasts from human, rhesus macaque, mouse and rat, stimulated with dsRNA (poly I:C) in a time course of 0,2,4 and 8 hours, profiled using the Smart-seq2 protocol.
The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.

INSTRUMENT(S): Illumina HiSeq 2500, Becton Dickinson INFLUX

ORGANISM(S): Musculus  

SUBMITTER: Tzachi Hagai  

PROVIDER: E-MTAB-5920 | ArrayExpress | 2018-07-06

SECONDARY ACCESSION(S): ERP024341

REPOSITORIES: ArrayExpress, ENA

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Publications


As the first line of defence against pathogens, cells mount an innate immune response, which varies widely from cell to cell. The response must be potent but carefully controlled to avoid self-damage. How these constraints have shaped the evolution of innate immunity remains poorly understood. Here we characterize the innate immune response's transcriptional divergence between species and variability in expression among cells. Using bulk and single-cell transcriptomics in fibroblasts and mononuc  ...[more]

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