Transcriptomics

Dataset Information

346

Single-cell RNA-sequencing of Platynereis dumerilii


ABSTRACT: For unbiased, whole-organism wide cell type profiling, we randomly sampled cells from dissociated Platynereis larvae. To generate the single-cell mRNA-sequencing data, P. dumerilii larvae were dissociated, followed by cell capture, cDNA synthesis and amplification on the C1 Single-Cell Auto Prep IFCs for 5-10 um or 10-17 um cells (Fluidigm). Sequencing libraries were produced using Nexera XT DNA kit (Illumina). In total, we sequenced 596 samples, of which 373 correspond to single, alive cells that passed the quality check criteria. Part of this dataset was previously published (ArrayExpress accession number E-MTAB-2865). Here, we publish additional 383 sequenced cells.

INSTRUMENT(S): C1 Single-Cell Auto Prep System, Fluidigm, Illumina HiSeq 2000

ORGANISM(S): Platynereis dumerilii  

SUBMITTER: Nils Eling   John C Marioni   Kaia Achim  

PROVIDER: E-MTAB-5953 | ArrayExpress | 2017-09-18

SECONDARY ACCESSION(S): ERP104242

REPOSITORIES: ArrayExpress, ENA

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