Single-cell RNA-seq of of dermal fibroblasts using microfluidic droplet capture (unstimulated cells)
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ABSTRACT: The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.
This experiment contains data of dermal fibroblasts from 3 human individuals, unstimulated, sequenced by 10X Genomics technology. Corresponding data from stimulated cells are found under ArrayExpress accession E-MTAB-5989.
INSTRUMENT(S): Illumina HiSeq 2500
ORGANISM(S): Homo sapiens
SUBMITTER:
Tzachi Hagai
PROVIDER: E-MTAB-5988 | ArrayExpress | 2018-07-06
SECONDARY ACCESSION(S): ERP024405
REPOSITORIES: ArrayExpress, ENA
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