Transcriptomics

Dataset Information

25

Examination of tumor samples obtained after laparoscopic supracervical hysterectomy and a derivative leiomyosarcoma


ABSTRACT: Unexpected malignant tumors are a rare finding after surgery for symptomatic leiomyomas but there is little doubt that morcellation of these lesions is associated with a higher risk of iatrogenic peritoneal spread compared to women having surgery without morcellation. Thus, the FDA has issued a warning against the use of power morcellation in the majority of women undergoing myomectomy or hysterectomy for treatment of fibroids. We present a case report of 50 year old patient with intervertebral disc degeneration and multiple uterine fibroids decided to have laparoscopic supracervical hysterectomy. 28 months later the patient cystic adnexal mass removed by laparotomy and histologically, classified as leiomyosarcoma (sample H_12814-15). 19 months after this latter surgery MRI revealed a 10 x 8 x 6 cm abdominal mass attached to the liver and numerous other nodules attached to the abdominal wall. We investigated genomic profiles of four samples obtained from initial surgery and the malignant tumor that appeared 16 month later by molecular inversion probe (MIP) array hybridization. DNA was extracted from FFPE Tissue Samples using Covaris adaptive focused acoutics (AFATM) and truXTRACTM FFPE DNA kit and subjected to CGH analysis using the Affymetrix OncoScan platform according to the manufacturers protocol.

INSTRUMENT(S): GeneChipR Scanner 3000, Covaris M220, GeneChipR Hybridization Oven 645, GeneChip Fluidics Station 450

ORGANISM(S): Homo sapiens  

SUBMITTER: Carsten Holzmann  

PROVIDER: E-MTAB-6039 | ArrayExpress | 2018-04-30

REPOSITORIES: ArrayExpress

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