Dataset Information


Single-cell RNA-seq of dermal fibroblasts stimulated with dsRNA (poly I:C) in a time course of 0, 4 and 8 hours

ABSTRACT: Dermal fibroblasts from human, rhesus macaque, mouse and rat, stimulated with dsRNA (poly I:C) in a time course of 0,4 and 8 hours, profiled using the Smart-seq2 protocol.
The innate immune response - the expression programme that is initiated once a pathogen is sensed - is known to be variable among responding cells, as well as to rapidly evolve in the course of mammal evolution. To study the transcriptional divergence and cell-to-cell variability of this response, we stimulated dermal fibroblast cells from two primates (human and macaque) and two rodents (mouse and rat) with dsRNA - a mimic of viral RNA that elicits a rapid innate immune response. Subsequently, we profiled the response using bulk RNA-seq, scRNA-seq and ChIP-seq across the four species and across different time points.

INSTRUMENT(S): Illumina HiSeq 2500, Beckman Coulter MoFlo XDP

ORGANISM(S): Homo Sapiens Macaca Mulatta Rattus Norvegicus Mus Musculus

DISEASE(S): Normal

SUBMITTER: Tzachi Hagai  

PROVIDER: E-MTAB-6066 | ArrayExpress | 2018-07-06



Dataset's files

Action DRS
E-MTAB-6066.idf.txt Idf Processed
E-MTAB-6066.sdrf.txt Txt
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