Transcriptomics

Dataset Information

0

Single cell RNA sequencing of stem cell-derived retinal ganglion cells


ABSTRACT: We used human embryonic stem cell-derived retinal ganglion cells (RGCs) to characterize the transcriptome of 1,174 cells at the single cell level. The human embryonic stem cell line BRN3B-mCherry A81-H7 was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and incubated with THY1 antibody (Miltenyi) before undergoing FACS. THY1 positive and THY1 negative cells were subsequently prepared for single cell RNA sequencing. Single cell suspensions were loaded onto 10X Genomics Single Cell 3' Chips along with the reverse transcription master mix as per the manufacturer's protocol for the Chromium Single Cell 3' v2 Library (10X Genomics; PN-120233), to generate single cell gel beads in emulsion. Libraries were then sequenced on an Illumina HiSeq 2500.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens  

SUBMITTER: Joseph E Powell   Alice Pébay   Alex W Hewitt  

PROVIDER: E-MTAB-6108 | ArrayExpress | 2017-10-07

SECONDARY ACCESSION(S): ERP108448

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications


We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster  ...[more]

Similar Datasets

2019-05-12 | E-MTAB-7919 | ArrayExpress
2018-05-30 | E-MTAB-6677 | ArrayExpress
2018-08-17 | E-MTAB-6687 | ArrayExpress
2019-05-11 | E-MTAB-7918 | ArrayExpress
2018-06-25 | E-MTAB-6854 | ArrayExpress
2018-07-09 | E-MTAB-6429 | ArrayExpress
2018-10-02 | E-MTAB-6701 | ArrayExpress
2018-03-01 | E-MTAB-6411 | ArrayExpress
2017-11-21 | E-MTAB-6173 | ArrayExpress
2018-12-05 | E-MTAB-6967 | ArrayExpress