Transcriptomics

Dataset Information

345

Comparison of the metabolic response to over-production of p-coumaric acid in two yeast strains


ABSTRACT: We aimed to study how production of p-coumaric acid, a precursor of multiple secondary aromatic metabolites, influences the cellular metabolism of Saccharomyces cerevisiae. We evaluated the growth and p-coumaric acid production in batch and chemostat cultivations and analyzed the transcriptome and intracellular metabolome during steady state in low- and high-producers of p-coumaric acid in two strain backgrounds, S288c or CEN.PK. For analysis of the differential gene expression, we did pairwise comparisons between the optimized and non-optimized strains for p-CA production: CEN.PK strains (ST4288 and ST4408) and the S288c strains (ST4353 and ST4397). Transcriptome analysis showed that the CEN.PK strain was less affected by engineering towards higher p-CA production than the S288c strain, as the number of significantly up-/down-regulated genes was correspondingly 652 and 1927 amongst others, strain S288c had downregulations in gene sets involved in amino acid and protein biosynthesis. This suggests that CEN.PK may be a better platform strain for production of aromatic compounds than the S288c strain.

INSTRUMENT(S): Computerome, NextSeq 500

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Irina Borodina   Emre Ozdemir   Angelica Rodriguez  

PROVIDER: E-MTAB-6208 | ArrayExpress | 2017-11-21

SECONDARY ACCESSION(S): ERP105270

REPOSITORIES: ArrayExpress, ENA

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Publications

Comparison of the metabolic response to over-production of p-coumaric acid in two yeast strains.

Rodriguez Angelica A   Chen Yun Y   Khoomrung Sakda S   Özdemir Emre E   Borodina Irina I   Nielsen Jens J  

Metabolic engineering 20171031


The development of robust and efficient cell factories requires understanding of the metabolic changes triggered by the production of the targeted compound. Here we aimed to study how production of p-coumaric acid, a precursor of multiple secondary aromatic metabolites, influences the cellular metabolism of Saccharomyces cerevisiae. We evaluated the growth and p-coumaric acid production in batch and chemostat cultivations and analyzed the transcriptome and intracellular metabolome during steady  ...[more]

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