Transcriptomics

Dataset Information

42

Whole-Genome Gene Expression Bead Array analysis of Toll-like receptor 2/8 stimulated human monocytes


ABSTRACT: Monocytes and macrophages are essential cells in the early response in their capacity as ubiquitous phagocytic cells. They phagocytose microorganisms or damaged cells and sense pathogen/damage-associated molecular patterns (PAMPs/DAMPs) through innate receptors such as Toll-like receptors (TLRs). We investigated a phenomenon where co-signaling from TLR2 and TLR8 in human primary monocytes provides a distinct immune activation profile compared to signaling from either TLR alone. Comparison of gene signatures induced by either stimulus alone or together, show that co-signaling result in downstream differences in regulation of signaling and gene transcription. To investigate the interaction of TLR2 and TLR8 stimulation primary human monocytes isolated from buffy coat were stimulated with TLR2/6 ligand FSL-1 and/or TLR7/8 ligand CL075 and the gene expression monitored at 1,2 and 3h post stimulation.

ORGANISM(S): Homo sapiens  

SUBMITTER: Richard K Kandasamy   Bjarte Bergstrøm   Korbinian Bösl   Terje Espevik  

PROVIDER: E-MTAB-6222 | ArrayExpress | 2018-03-09

REPOSITORIES: ArrayExpress

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Publications

Coactivation of TLR2 and TLR8 in Primary Human Monocytes Triggers a Distinct Inflammatory Signaling Response.

Bösl Korbinian K   Giambelluca Miriam M   Haug Markus M   Bugge Marit M   Espevik Terje T   Kandasamy Richard K RK   Bergstrøm Bjarte B  

Frontiers in physiology 20180529


Innate immune signaling is essential to mount a fast and specific immune response to pathogens. Monocytes and macrophages are essential cells in the early response in their capacity as ubiquitous phagocytic cells. They phagocytose microorganisms or damaged cells and sense pathogen/damage-associated molecular patterns (PAMPs/DAMPs) through innate receptors such as Toll-like receptors (TLRs). We investigated a phenomenon where co-signaling from TLR2 and TLR8 in human primary monocytes provides a d  ...[more]

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