Genomics

Dataset Information

26

Systematic identification of G-quadruplex sensitive lethality by genome-wide genetic screening


ABSTRACT: A375 and HT1080 cells are treated with G-quadruplex ligands PDS or PhenDC3 following genome-wide shRNA knockdown. This enables the identification of genes that when silenced, specifically compromises cell growth in the presence of the ligand. First, a pilot screen was performed to determine a ligand concentration and experimental duration that caused ligand-specific, significant changes in shRNA levels. Second, a genome-wide screen was performed to globally evaluate G4-ligand synthetic lethal interactions. Third, to corroborate the G4-sensitisers uncovered in the genome-wide screen, a focussed screen was performed with a custom shRNA pool.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens  

SUBMITTER: Sergio Martínez Cuesta   Shankar Balasubramanian   Darcie S. Mulhearn   Katie G. Zyner  

PROVIDER: E-MTAB-6367 | ArrayExpress | 2019-06-22

SECONDARY ACCESSION(S): ERP106217

REPOSITORIES: ArrayExpress, ENA

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