Dataset Information


Massively parallel single-cell RNA-sequencing of K14E7 transgenic and wildtype C57BL/6 mice

ABSTRACT: We report scRNA-seq data captured from 9,410 cells obtained from the skin of K14E7 transgenic and wildtype C57/BL6 mice. The K14E7 mouse model harbors the HPV16 E7 oncogene driven from a Keratin 14 promoter for keratinocyte-specific expression. We used scRNA-seq to detect and measure E7 transcription with unprecedented accuracy and resolution. With these data, we uncovered transcriptional differences between the individual cells; demonstrated that increased HPV16 E7 copy number is associated with increased expression of E7-induced genes; and showed that E7 expression is predominantly associated with basal keratinocytes.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus  

SUBMITTER: Samuel Lukowski   Ian Frazer  

PROVIDER: E-MTAB-6429 | ArrayExpress | 2018-07-09



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Detection of HPV E7 Transcription at Single-Cell Resolution in Epidermis.

Lukowski Samuel W SW   Tuong Zewen K ZK   Noske Katharina K   Senabouth Anne A   Nguyen Quan H QH   Andersen Stacey B SB   Soyer H Peter HP   Frazer Ian H IH   Powell Joseph E JE  

The Journal of investigative dermatology 20180630 12

Persistent human papillomavirus (HPV) infection is responsible for at least 5% of human malignancies. Most HPV-associated cancers are initiated by the HPV16 genotype, as confirmed by detection of integrated HPV DNA in cells of oral and anogenital epithelial cancers. However, single-cell RNA sequencing may enable prediction of HPV involvement in carcinogenesis at other sites. We conducted single-cell RNA sequencing on keratinocytes from a mouse transgenic for the E7 gene of HPV16 and showed sensi  ...[more]

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