Genomics

Dataset Information

331

Targeted sequencing of 49 primary medulloblastoma samples using Illumina TruSight Tumor 15 assay


ABSTRACT: The mutation status of TP53 was analyzed by next generation sequencing using the Illumina TruSight Tumor (TST) 15 assay (Illumina) at the Molecular Pathology Center of the Jewish General Hospital (Montreal, QC, Canada). The CAP-compliant clinically validated TST15 assay was performed as per supplier's instruction with 10 ng DNA input. Libraries were sequenced on a MiSeq with a v3 flow cell (Illumina). Data was analyzed with a clinically validated pipeline (NextGene, SoftGenetics) and variants were annotated in Geneticist Assistant (SoftGenetics). The IARC TP53 database version R18 was consulted for the molecular interpretation of TP53 variants (www.p53.iarc.fr).

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Homo sapiens  

SUBMITTER: Walderik Zomerman  

PROVIDER: E-MTAB-6546 | ArrayExpress | 2018-03-20

SECONDARY ACCESSION(S): ERP107161

REPOSITORIES: ArrayExpress, ENA

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