Transcriptomics

Dataset Information

51

Transcription profiling by array of Arabidopsis thaliana Col-0 and rcd1 genotypes after ozone and control treatments


ABSTRACT: Wild-type Columbia (Col-0) and rcd1-1 seeds were sown on 1:1 peat:vermiculite mixture, stratified for 2 days and grown in controlled environment chambers (Weiss Bio1300; Weiss Gallenkamp, (http://www.weiss-gallenkamp.com/) with 12-h/12-h day/night cycle, temperature 22 °C/19 °C, relative humidity 70%/90%. 1-week old plants were transplanted into individual pots (5x5cm) and subirrigated twice a week. O3 experiments (6 hours of 350 nL L-1) were performed with 3-week old plants. Control and O3-treated plants (x-10 individuals) were collected at 0, 1, 2, 4, 8 and 24h after the start of the O3 treatment. The experiment was repeated 3 times, in addition to which a fourth identical repeat was used as the common reference RNA. cDNA synthesis, sample labeling, microarray hybridization, washing of the slides as well as image scanning were performed as described in Jaspers et al. (2009; PMID:19548978). The 50-mer oligo microarrays were identical to the Jaspers et al. 2009. In prerocessing, the 50mer probes were reannoated to TAIR9 with BLASTn algorithm. Data was analysed with linear mixed models using scripts in R.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Jarkko T Salojarvi  

PROVIDER: E-MTAB-662 | ArrayExpress | 2011-06-30

REPOSITORIES: ArrayExpress

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Publications


Plant responses to changes in environmental conditions are mediated by a network of signaling events leading to downstream responses, including changes in gene expression and activation of cell death programs. Arabidopsis thaliana RADICAL-INDUCED CELL DEATH1 (RCD1) has been proposed to regulate plant stress responses by protein-protein interactions with transcription factors. Furthermore, the rcd1 mutant has defective control of cell death in response to apoplastic reactive oxygen species (ROS).  ...[more]

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