Project description:RBP9-TAP was expressed in bloodstream T. brucei and bound mRNA was pulled down using IgG beads and eluted via TEV-protease cleavage. rRNA was depleted using RNAseH and rRNA hybridizing oligos.

2020-05-30 | E-MTAB-9093 | ArrayExpress

RNAseq of DRBD7-TAP interacting RNAs in Trypanosoma brucei

Project description:DRBD7 interacting mRNAs were identified using a DRBD7-TAP fusion protein for protein pulldown. To study enrichment, the flow-through was also subjected to RNAseq.

2020-05-30 | E-MTAB-9092 | ArrayExpress

RNA-Seq data: RNA-IP (TAP-ZC3H30 pull down followed by RNA-Seq) and total transcriptomics (Effect of ZC3H30 gene deletion) in procyclic form of T. brucei

Project description:The first 4 samples belong to the RNA-IP using in situ TAP tagged ZC3H30 in procyclic (insect) form of the parasite T. brucei Lister 427, 2 samples are Elu or eluate, and 2 are FL or flowthrough (unbound) sample. The other 8 samples are also from procyclic cells. 4 samples belong to DKO(ZC3H30 gene double knockout), 2 are non-stressed and 2 are heat shocked samples; the rest 4 samples are DKO-ectopic (ZC3H30 double knockouts, expressing, ectopic copy of ZC3H30) 2 are non-stressed and 2 are heat shocked samples. Heat Shock experiment was done at 39 degree Celsius.

2017-12-16 | E-MTAB-6281 | ArrayExpress

RNAseq of ZC3H5 interacting mRNA

Project description:Bloodstream-form trypanosomes (Lister 427) constitutively expressing ZC3H5-TAP (Tb927.3.740) were used. The protein was pulled down with an IgG column , then the protein and bound RNA was eluted using TEV protease. RNA was then sequenced from unbound (flow-through) and bound (eluate) fractions.

2020-06-06 | E-MTAB-9084 | ArrayExpress

Function of Trypanosome brucei RBP10

Project description:RBP10 RNAi, ectopic expression or RBP10-myc and RNA IP using TAP-RBP10

2016-06-27 | E-MTAB-4564 | ArrayExpress