Transcriptomics

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Single cell RNA-sequencing of primary mouse Neural Plate Border Stem Cells


ABSTRACT: We describe a so far uncharacterized, embryonic and self-renewing Neural Plate Border Stem Cell (NBSC) population with the capacity to differentiate into central nervous and neural crest lineages. NBSCs can be obtained by neural transcription factor-mediated reprogramming (BRN2, SOX2, KLF4, and ZIC3) of human adult dermal fibroblasts and peripheral blood cells (induced Neural Plate Border Stem Cells, iNBSCs) or by directed differentiation from human induced pluripotent stem cells (NBSCs). Moreover, human (i)NBSCs share molecular and functional features with an endogenous NBSC population isolated from neural folds of E8.5 mouse embryos. Upon differentiation, iNBSCs give rise to either (1) radial glia-type stem cells, dopaminergic and serotonergic neurons, motoneurons, astrocytes, and oligodendrocytes or (2) cells from the neural crest lineage. Here we provide single cell RNA-sequencing data of two primary mouse Neural Plate Border Stem Cell Lines (pNBSCs). pNBSCs were single cell sorted and RNA sequencing was performed following the Smart-seq2 protocol. In sum, pNBSCs and iNBSCs share a similar regional identity, expression signature and analogous differentiation dynamics on the single-cell-level, suggesting the presence of a transient, NBSC-like progenitor during the neurulation stage of mouse and likely also human embryos.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus  

SUBMITTER: Andreas Trumpp  Marc Christian Thier     

PROVIDER: E-MTAB-6925 | ArrayExpress | 2018-12-19

SECONDARY ACCESSION(S): ERP109372

REPOSITORIES: ArrayExpress, ENA

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