Transcriptomics

Dataset Information

30

Single-cell RNA sequencing of mouse germ cells


ABSTRACT: Spermatogenesis is a recurring differentiation process that results in the production of male gametes within the testes. During this process, spermatogonial stem cells differentiate to form spermatocytes, which undergo two rounds of meiotic division to form haploid spermatids. Throughout spermiogenesis, round spermatids elongate to form mature sperm. To profile maturing germ cells during the first wave of spermatogenesis, we generated droplet-based single cell RNAseq from juvenile animals at post-natal day P5-P35 as well as adult animals. Cells were isolated from whole testes. Furthermore, to assay the robustness of the meiotic cell division process, we profiled germ cells of the trans-chromosomal mouse model (Tc1) that carries a copy of the human chromosome 21.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus  

SUBMITTER: Nils Eling  

PROVIDER: E-MTAB-6946 | ArrayExpress | 2019-01-25

SECONDARY ACCESSION(S): ERP109525

REPOSITORIES: ArrayExpress, ENA

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Publications

Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis.

Ernst Christina C   Eling Nils N   Martinez-Jimenez Celia P CP   Marioni John C JC   Odom Duncan T DT  

Nature communications 20190319 1


Male gametes are generated through a specialised differentiation pathway involving a series of developmental transitions that are poorly characterised at the molecular level. Here, we use droplet-based single-cell RNA-Sequencing to profile spermatogenesis in adult animals and at multiple stages during juvenile development. By exploiting the first wave of spermatogenesis, we both precisely stage germ cell development and enrich for rare somatic cell-types and spermatogonia. To capture the full co  ...[more]

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