Transcriptomics

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Full transcriptome analysis of MCF-7 cells treated with screening hit compounds versus control


ABSTRACT: This experiment was performed as part of a hit screening project. Transcriptome analysis was performed to determine whether the screened hits induced a gene expression pattern consistent with proteasome inhibition. MCF-7 cells were exposed to 2x[IC50] of hit compounds for six hours. RNA was processed according to manufacturer's protocol for preparation of HuGene 2.0ST chips to acquire biotinylated sense-strand DNA. Biotinylated DNA was hybridized to HuGene 2.0ST chips at 37°C for 16 hours in a GeneChip® Hybridization Oven 645, washed and stained using the GeneChip® Fluidics Station 450 using the FS450_0002 protocol and scanned with a GeneChip® Scanner 3000 7G.

ORGANISM(S): Homo sapiens  

SUBMITTER: Arjan Mofers   

PROVIDER: E-MTAB-7024 | ArrayExpress | 2018-08-04

REPOSITORIES: ArrayExpress

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