Transcriptomics

Dataset Information

297

RNA-Seq of yeast FBY107, FBY106 strains to investigate pre-mRNA degradation pathway


ABSTRACT: General discard pathways eliminate unprocessed and irregular pre-mRNAs to control the quality of gene expression. In contrast to such general pre-mRNA decay, we describe here a nuclear pre-mRNA degradation pathway that controls the expression of select intron-containing genes. We show that the fission yeast nuclear poly(A)-binding protein, Pab2, and the nuclear exosome subunit, Rrp6, are the main factors involved in this polyadenylation-dependent pre-mRNA degradation pathway. Transcriptome analysis and intron swapping experiments revealed that inefficient splicing is important to dictate susceptibility to Pab2-dependent pre-mRNA decay. We also show that negative splicing regulation can promote the poor splicing efficiency required for this pre-mRNA decay pathway, and in doing so identify a mechanism of cross-regulation between paralogous ribosomal proteins through nuclear pre-mRNA decay. Our findings unveil a layer of regulation in the nucleus in which the turnover of specific pre-mRNAs, besides the turnover of mature mRNAs, is used to control gene expression.

INSTRUMENT(S): Illumina Genome Analyzer II, Illumina Genome Analyzer IIx

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Francois Bachand   Jürg Bähler   Samuel Marguerat  

PROVIDER: E-MTAB-708 | ArrayExpress | 2012-02-10

SECONDARY ACCESSION(S): ERP000759

REPOSITORIES: ArrayExpress, ENA

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