Dataset Information


ChIP-seq of Pbx1, Prep1 and Sp2 in WT MEFs, Sp2ko MEFs and in Sp2ko MEFs ectopically expressing Flag-tagged full length Sp2; and ChIPexo of Sp2 in WT and Sp2ko MEFs

ABSTRACT: We investigated the genomic binding profiles of the transcription factors Pbx1, Prep1, and Sp2 in mouse embryonic fibroblasts using ChIPseq and ChIPexo. Refer to E-MTAB-2970 and E-MTAB-994 for KO and IgG Controls.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Mus musculus  

SUBMITTER: Florian Finkernagel   Guntram Suske  

PROVIDER: E-MTAB-7125 | ArrayExpress | 2018-10-17



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Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites.

Völkel Sara S   Stielow Bastian B   Finkernagel Florian F   Berger Dana D   Stiewe Thorsten T   Nist Andrea A   Suske Guntram G  

The Journal of biological chemistry 20181018 50

Different transcription factors operate together at promoters and enhancers to regulate gene expression. Transcription factors either bind directly to their target DNA or are tethered to it by other proteins. The transcription factor Sp2 serves as a paradigm for indirect genomic binding. It does not require its DNA-binding domain for genomic DNA binding and occupies target promoters independently of whether they contain a cognate DNA-binding motif. Hence, Sp2 is strikingly different from its clo  ...[more]

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