Transcriptomics

Dataset Information

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RNA-seq of etiolated 9 day old maize seedling second leaves after exposure to either blue, red or no light conditions for three hours


ABSTRACT: Characterisation of the differences in transcript abundance between maize leaves that have been exposed to blue, red, or no light: B73 maize seeds were planted and grown in the dark for 9 days. Etiolated second leaves were clamped 5 cm from the leaf tip by a Licor 6800 device equipped with a multi-phase flash fluorometer head, which administered 100 µmol m-2s-1 of either 100% red or 100% blue light. The Licor was configured with flow rate 500 µmol s⁻¹, 400 µmol mol⁻¹ CO2, leaf temperature 28°C and 60% humidity. Fluorescence and gas exchange were measured every 15 minutes for 3 hours. Leaf samples were collected between 12.30am-2pm each day.

INSTRUMENT(S): BGISEQ-500

ORGANISM(S): Zea mays  

SUBMITTER: Steve Kelly   Ross-W. S Hendron  

PROVIDER: E-MTAB-7200 | ArrayExpress | 2019-06-10

SECONDARY ACCESSION(S): ERP115257

REPOSITORIES: ArrayExpress, ENA

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