Transcriptomics

Dataset Information

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RNA-Seq data of terminal differentiated cells in Dictyostelium discoideum


ABSTRACT: The intent of the experiment was to gather transcriptomic data from terminally differentiated cell types --spore, stalk, and cup cells-- in a simple multicellular model system, Dictyostelium discoideum, in order to characterize the cell-type specific gene expression patterns. Specifically, we dissociated and collected each cell type from the fruiting bodies of D. discoideum at 24 hours of development. We also collected exponentially growing vegetative cells of D. discoideum.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Dictyostelium discoideum  

SUBMITTER: Koryu Kin   Pauline Schaap  

PROVIDER: E-MTAB-7215 | ArrayExpress | 2019-01-31

SECONDARY ACCESSION(S): ERP110995

REPOSITORIES: ArrayExpress, ENA

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Publications

Cell-type specific RNA-Seq reveals novel roles and regulatory programs for terminally differentiated Dictyostelium cells.

Kin Koryu K   Forbes Gillian G   Cassidy Andrew A   Schaap Pauline P  

BMC genomics 20181022 1


BACKGROUND:A major hallmark of multicellular evolution is increasing complexity by the evolution of new specialized cell types. During Dictyostelid evolution novel specialization occurred within taxon group 4. We here aim to retrace the nature and ancestry of the novel "cup" cells by comparing their transcriptome to that of other cell types. RESULTS:RNA-Seq was performed on purified mature spore, stalk and cup cells and on vegetative amoebas. Clustering and phylogenetic analyses showed that cup  ...[more]

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