Transcriptomic responses of two population strains of the kelp Saccharina latissima to temperature and salinity changes
ABSTRACT: The data is a result of a large laboratory experiment targeting interactive effects of salinity and temperature on the transcriptomic level in algae from two populations of an ecologically relevant kelp species (Laminariales), Saccharina latissima. Research interest in S. latissima has recently been increasing given its importance as ecosystem engineer along temperate rocky shores in the Atlantic Ocean, and its growing potential in industrial applications such as aquaculture, pharmaceutics, food and feed. Young sporophytes of S. latissima were raised from stock cultures of clonal male and female gametophytes at the Alfred-Wegener-Institute Helmholtz Centre for Polar and Marine Research. Cultures originated from sporophyte collected at Kongsfjorden (79°N, 11°E; Spitsbergen, Norway) and at Roscoff (48° 43′ 39″ N, 3° 59′ 13.2″ W; Brittany, France). Sporophytes of both populations were grown aerated in glass beakers at 8°C and under a photon fluence rate of 20 µmol photons m–2 s–1 of photosynthetically active radiation with a 18 h light: 6 h dark photoperiod and were cultivated in sterile seawater enriched with Provasoli (Starr & Zeikus 1993) with an absolute salinity (SA) of ~30 during three months. At the start of the experiment, sporophytes were either kept at 8°C (or transferred to 0°C and 15°C) in temperature controlled rooms. After one week, per each temperature, sporophytes were divided into a low salinity treatment of SA 20 or kept at the control salinity (SA 30).
Project description:To explore possible interactive effects of UV-radiation, temperature and growth conditions, cultivated and field sporophytes of Saccharina latissima were exposed for 24h to UV-radiation at three different temperatures (2,7 & 12°C). Gene expression profiles under UV-radiation at different temperatures were assessed through microarray hybridizations, afterwards comparisons of gene expression profiles in field and culture sporophytes were carried out.
Project description:We performed a laboratory experiment with vegetative gametophytes of the kelp Saccharina latissima and exposed the gametophytes to three temperatures (4°C, 12°C and 20°C) by sex (female, male) for 14 days.
Project description:Effect of PAR and temperature stress on the gene expression Saccharina latissima. Total RNA of stress treatments (low PAR 2° and 17°C, high PAR 2° and 17°C) was hybridized against the control treatment (low PAR 12°C); hybridizations were carried out in 4 replicates.
Project description:Sporophytes of Saccharina latissima were exposed for two weeks to 12 different combinations of photosynthetically active radiation and UVR at three different temperatures (2,7,12 C). Maximum quantum yield of photosystem II was determined twice a week during the experimental duration for observing the extent of photoinhibition. For investigating molecular mechanisms of acclimation to high photosynthetically radiation, and UVR gene expression profiles were assessed through microarray hybridizations.
Project description:Salinity is a major factor that can affect greatly the viability of the fish larvae during their developing stages. Natural populations are exposed to high fluctuation in the environmental conditions. Also, salinity management is of high relevance in hatcheries. The objective of this study was to determine the biological effects of environmental salinities on larval development in sole. Larvae were exposed to two salinities, 10 and 36 ppt, from eggs until the 3 days after hatching. Expression profiles were determined using RNA-seq, microarray and validated using qPCR. Important malformations were identified. The identification of key molecular pathways affected by salinity can help to understand the effects of salinity during early developmental stages with a profound impact to evaluate the effects of climate change and improve hatchery practices. Overall design: Fertilized eggs of Senegalese sole were collected from spontaneous spawn at “El Toruño” facilities (El Puerto de Santa María, Cadiz). Temperature in the broodstock during the spawning was approximately 18.5ºC and salinity 34 ppt. Eggs were transferred to a 1,000-mL measuring cylinder to separate buoyant (viable) from non-buoyant (non-viable) eggs and the number in each fraction was estimated using volumetric methods (1,100 eggs mL-1). After estimating the number of fertilized eggs, we incubated embryos (at the gastrula stage) in 15-L cylinder tanks at an initial density of 2,000 embryos L-1. After seeding, two salinities (10 and 36 ppt) were established using a recirculation system that kept constant temperature (20ºC±0.5) and target salinity. Water turnover was maintained at one total renewal per hour during the experiment. Trial was done in duplicate tanks for each salinity. After hatching, larvae were sampled at day 3 using a 350 µm-mesh net. Three pools of larvae were collected from each tube (~100 larvae/pool), washed with DEPC water, frozen directly in liquid nitrogen and stored at -80 °C until analysis.
Project description:To understand the sensitivity of larval lice to changing environmental parameters we applied a 38K oligo microarray and characterized transcriptome responses after 24 hour exposures to reduced salinity (30-10 parts per thousand (ppt)) or varied temperature (16-4ºC). To further characterize the effects of salinity, we profiled expression changes over a range of seawater with single increment differences (30-25 ppt). Three separate projects. Low resolution salinity (aka wide range or LR_SAL) contains 2 duplicate experiments, each with pools of lice (~500 copepodid lice per beaker; n=3 beakers per condition), incubated for 24 hr at 10ºC diluted to 30, 25, 20, or 10 parts per thousand (ppt). Temperature study conducted as above, but with a constant salinity of 30 ppt and changed temperature of 4, 10, or 16ºC. High resolution salinity conducted as above, but with no experiment replication, but with biological repication of n=6 beakers per condition, and with a range of 25, 26, 27, 28, 29, 30 ppt salinity. Please note that each experiment was normalized separately.
Project description:Salicylic acid (SA) levels increase in Arabidopsis upon exposure to low temperature for more than a week. This cold-induced SA biosynthesis was found to proceed through the isochorismate synthase (ICS) pathway. The sid2-1 mutant is deficient in ICS1 and does not make SA at low temperature. We used microarray analysis to examine the genes differentially regulated by low temperature that were dependent on SA We used the sid2-1 mutant and a WT control at 22⁰C and after 3 weeks at 4⁰C. All samples were in triplicate.
Project description:Sea cucumber, Apostichopus japonicus is a very important species for aquaculture, and its behavior and physiology can respond to the initial change in salinity. It is important to understand the molecular responses of A. japonicus when exposed to ambient changes in salinity In this study, RNA-seq provided a general overview of the gene expression profiles of the intestine of A. japonicus exposed to high salinity (SD40), normal salinity (SD30) and low salinity (SD20) environment. Overall design: Sea cucumbers were acclimated in tanks containing aerated sand-filtered seawater (salinity 30%, pH 8.0) at 15 ± 0.5°C for one week before use and were fed once each day at 8:00 am during this period. The animals were then assigned randomly to three groups (20 individuals in each group). One group of sea cucumbers was maintained as the control group with normal salinity (SD30) of around 30, whereas the other two groups were kept in sea water where the salinity was gradually increased and decreased to high salinity (SD40) of around 40 and low salinity (SD20) of around 20 at a rate of 2.5 per 6 h.