Transcriptomics

Dataset Information

0

Ribosome profiling of chondrocytic cell line SW1353 following exposure to IL-1B


ABSTRACT: Osteoarthritis is a chronic disease characterised by the loss of articular cartilage in synovial joints through a process of extracellular matrix destruction that is strongly associated with inflammatory stimuli. Chondrocytes undergo changes to their protein translation capacity during osteoarthritis, but a study of how disease-relevant signals effect chondrocyte protein translation at the transcriptomic level has not previously been performed. In this study we describe how the inflammatory cytokine interleukin 1-beta (IL-1β) rapidly affects protein translation in the chondrocytic cell line SW1353. Using ribosome profiling we demonstrate that IL-1β induced altered translation of inflammatory-associated transcripts such as NFKB1, TNFAIP2, MMP13, CCL2 and CCL7, as well as a number of ribosome-associated transcripts, through differential translation and the use of multiple open reading frames. Proteomic analysis of the cellular layer and the conditioned media of these cells identified that proteins which were differentially translated were most readily detected in the secretome. These translationally regulated secreted proteins included a number of chemokines and cytokines, underlining the rapid, translationally-mediated inflammatory cascade that is initiated by IL-1 β.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens  

SUBMITTER: Ben T McDermott  

PROVIDER: E-MTAB-7466 | ArrayExpress | 2019-07-12

SECONDARY ACCESSION(S): ERP112487

REPOSITORIES: ArrayExpress, ENA

altmetric image

Publications

Translational regulation contributes to the secretory response of chondrocytic cells following exposure to interleukin-1β.

McDermott Benjamin T BT   Peffers Mandy J MJ   McDonagh Brian B   Tew Simon R SR  

The Journal of biological chemistry 20190712 35


Osteoarthritis is a chronic disease characterized by the loss of articular cartilage in synovial joints through a process of extracellular matrix destruction that is strongly associated with inflammatory stimuli. Chondrocytes undergo changes to their protein translational capacity during osteoarthritis, but a study of how disease-relevant signals affect chondrocyte protein translation at the transcriptomic level has not previously been performed. In this study, we describe how the inflammatory c  ...[more]

Similar Datasets

2014-04-04 | E-GEOD-53693 | ArrayExpress
2015-02-13 | E-GEOD-62134 | ArrayExpress
2013-09-22 | E-GEOD-47558 | ArrayExpress
2013-09-26 | E-GEOD-51164 | ArrayExpress
2016-01-15 | E-GEOD-64962 | ArrayExpress
2014-08-28 | E-GEOD-53743 | ArrayExpress
2014-08-05 | E-GEOD-49716 | ArrayExpress
2013-09-26 | E-GEOD-51166 | ArrayExpress
2014-12-19 | E-GEOD-61742 | ArrayExpress
2015-07-23 | E-GEOD-66054 | ArrayExpress