Dataset Information


MiRNA profiling of Pax3-FOXO1-C2C12-derived exosomes compared to control C2C12 cells, using the Affymetrix GeneChip miRNA 3.0 array

ABSTRACT: C2C12 mouse myoblasts were transduced with either PAX3-FOXO1 expression vector (P3F-C2C12), a system that is commonly used to study the more aggressive alveolar rhabdomyosarcoma subtype, or empty vector (Ctrl-C2C12). Exosomes were isolated from both cell lines by differential centrifugation, and exosomal markers were characterized by western blot. Then, the Affymetrix GeneChip miRNA 3.0 array was used to identify the miRNA content of the extracted exosomes where the differentially deregulated miRNA (either enriched or depleted) in P3F-C2C12 exosomes were determined relative to Ctrl-C2C12 exosomes. Results showed that PAX3-FOXO1 fusion gene alters the content of exosomes and that the enriched miR-486-5p is a downstream effector of PAX3-FOXO1 in mediating the oncogenic effects of exosome-mediated paracrine signaling in this setting.

ORGANISM(S): Mus musculus  

SUBMITTER: Raya Saab   Sandra E. Ghayad  

PROVIDER: E-MTAB-7646 | ArrayExpress | 2019-12-01


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