Transcriptomics

Dataset Information

0

ScRNAseq of Cyp11a1-expressing cells from mouse melanoma model derived tumors


ABSTRACT: Tumours induce de novo steroidogenesis in immune cells. We wanted to define the gene expression identity of intratumoural steroidogenic immune cells and compare them with the transcriptome with non-steroidogenic cells. Cyp11a1 expression is a faithful biomarker of de novo steroidogenesis (i.e. Cyp11a1+ cells are steroidogenic and Cyp11a1- cells are non-steroidogenic). We inoculated B16-F10 tumor in Cyp11a1-mCherry reporter mice, enriched and purified intratumoral Cyp11a1-mCherry+ and Cyp11a1-mCherry- cells by cell sorting into 96-well plates [with a ratio of 79:15 (mCherry+ : mCherry-) cells per plate] and performed scRNA-seq using SMART-Seq2 platform.

INSTRUMENT(S): BD Influx, Illumina HiSeq 4000

ORGANISM(S): Mus musculus  

SUBMITTER: Krzysztof Polanski  

PROVIDER: E-MTAB-8509 | ArrayExpress | 2020-05-27

SECONDARY ACCESSION(S): ERP118399

REPOSITORIES: ArrayExpress, ENA

Similar Datasets

2020-01-01 | E-MTAB-8484 | ArrayExpress
2020-01-01 | E-MTAB-8486 | ArrayExpress
2020-10-14 | E-MTAB-9402 | ArrayExpress
2018-10-01 | E-MTAB-6116 | ArrayExpress
2010-09-21 | E-GEOD-21180 | ArrayExpress
2018-11-16 | E-MTAB-7117 | ArrayExpress
2014-09-24 | E-GEOD-61664 | ArrayExpress
2014-09-24 | E-GEOD-61661 | ArrayExpress
2014-09-24 | E-GEOD-61665 | ArrayExpress
2014-09-24 | E-GEOD-61662 | ArrayExpress