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DNA methylation profiling of epidermal tissue after acute repetitive irradiation

ABSTRACT: DNA methylation data generated from epidermal samples (suction blister roofs) of healthy female subjects after repetitive irradiation on three consecutive days using a full spectrum solar simulator. DNA was extracted from suction blisters taken from irradiated and control sites on the lower backs of each subject 24 hours after the final irradiation. DNA was bisulfite converted and then profiled using Illumina Infinium MethylationEPIC BeadChip arrays.

ORGANISM(S): Homo sapiens

DISEASE(S): Normal

SUBMITTER: Nicholas Holzscheck

PROVIDER: E-MTAB-9249 | ArrayExpress | 2020-09-02

REPOSITORIES: ArrayExpress

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			Action	DRS
	E-MTAB-9249.idf.txt	Idf
	E-MTAB-9249.idf.txt_original	Idf
	E-MTAB-9249.processed.1.zip	Processed
	E-MTAB-9249.raw.1.zip	Raw
	E-MTAB-9249.raw.2.zip	Raw

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Publications

Concomitant DNA methylation and transcriptome signatures define epidermal responses to acute solar UV radiation.

Holzscheck Nicholas N Söhle Jörn J Schläger Torsten T Falckenhayn Cassandra C Grönniger Elke E Kolbe Ludger L Wenck Horst H Terstegen Lara L Kaderali Lars L Winnefeld Marc M Gorges Katharina K

Scientific reports 20200731 1

The simultaneous analysis of different regulatory levels of biological phenomena by means of multi-omics data integration has proven an invaluable tool in modern precision medicine, yet many processes ultimately paving the way towards disease manifestation remain elusive and have not been studied in this regard. Here we investigated the early molecular events following repetitive UV irradiation of in vivo healthy human skin in depth on transcriptomic and epigenetic level. Our results provide fir ...[more]

PMID: 32737342

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RNA seq of epidermal tissue after acute repetitive irradiation

Project description:Whole transcriptome sequencing data generated from epidermal samples (suction blister roofs) of healthy female subjects after repetitive irradiation on three consecutive days using a full spectrum solar simulator. RNA was extracted from suction blisters taken from irradiated and control sites on the lower backs of each subject 24 hours after the final irradiation. RNA was cDNA converted and then sequenced on an Illumina HiSeq 2500 (single-end, 50 bp) to an approximate sequencing depth of 100 million reads per sample.

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