Dataset Information


ScRNA-seq analysis of cell subpopulations of the bronchoalveolar lavage fluid of healthy dogs

ABSTRACT: In dogs, a species for which markers of cell populations are often limiting, we sought to evaluate in an unbiased way the heterogeneity of cell subpopulations in the bronchoalveolar lavage fluid of healthy dogs, by single-cell RNA-sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Canis lupus familiaris  

SUBMITTER: Fastrès Aline  

PROVIDER: E-MTAB-9265 | ArrayExpress | 2020-06-29



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Characterization of the Bronchoalveolar Lavage Fluid by Single Cell Gene Expression Analysis in Healthy Dogs: A Promising Technique.

Fastrès Aline A   Pirottin Dimitri D   Fievez Laurence L   Marichal Thomas T   Desmet Christophe J CJ   Bureau Fabrice F   Clercx Cécile C  

Frontiers in immunology 20200730

Single-cell mRNA-sequencing (scRNA-seq) is a technique which enables unbiased, high throughput and high-resolution transcriptomic analysis of the heterogeneity of cells within a population. This recent technique has been described in humans, mice and other species in various conditions to cluster cells in populations and identify new subpopulations, as well as to study the gene expression of cells in various tissues, conditions and origins. In dogs, a species for which markers of cell population  ...[more]

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