Transcriptomics

Dataset Information

0

RNA-seq analysis of primary, processed and total RNAs of an E. coli wt and a mutant inactivated for RNase III


ABSTRACT: The experiment was aimed toward comparison of the abundance of total, primary and processed transcripts in E. coli strain inactivated for the RNase III relative to the wt strain. Analyzed RNAs were extracted from exponentially grown E. coli cultures at OD650 0.4 in LB medium at 37°C. 5' monophosphate (PSS fraction) and 5'triphosphate (TSS fraction) were tagged with specific primers. The untagged fraction is displayed as the INT fraction.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Escherichia coli K-12  

SUBMITTER: Eliane Hajnsdorf   Maxence Lejars  

PROVIDER: E-MTAB-9507 | ArrayExpress | 2020-10-15

SECONDARY ACCESSION(S): ERP123812

REPOSITORIES: ArrayExpress, ENA

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Publications

Landscape of RNA polyadenylation in E. coli.

Maes Alexandre A   Gracia Céline C   Innocenti Nicolas N   Zhang Kaiyang K   Aurell Erik E   Hajnsdorf Eliane E  

Nucleic acids research 20170301 5


Polyadenylation is thought to be involved in the degradation and quality control of bacterial RNAs but relatively few examples have been investigated. We used a combination of 5΄-tagRACE and RNA-seq to analyze the total RNA content from a wild-type strain and from a poly(A)polymerase deleted mutant. A total of 178 transcripts were either up- or down-regulated in the mutant when compared to the wild-type strain. Poly(A)polymerase up-regulates the expression of all genes related to the FliA regulo  ...[more]

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