Transcriptomics

Dataset Information

5

Transcription profiling of Salmonella enterica serotype SL1344 wildtype, pSf-R27sfh and pSf-R27 at stationary and exponential growth phases, to investigate the role of the Sfh protein encoded by transmissible plasmids and involved in human typhoid


ABSTRACT: The Sfh protein is encoded by self-transmissible plasmids involved in human typhoid and is closely related to the global regulator H-NS. We have found that Sfh provides a stealth function that allows the plasmids to be transmitted to new bacterial hosts with minimal effects on their fitness. Introducing the plasmid without the sfh gene imposes a mild H-NS- phenotype and a severe loss of fitness due to titration of the cellular pool of H-NS by the A+T-rich plasmid. This stealth strategy seems to be used widely to aid horizontal DNA transmission and has important implications for bacterial evolution.

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium  

SUBMITTER: Matloob Qureshi  

PROVIDER: E-SGRP-7 | ArrayExpress | 2007-01-15

REPOSITORIES: ArrayExpress

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Publications

An H-NS-like stealth protein aids horizontal DNA transmission in bacteria.

Doyle Marie M   Fookes Maria M   Ivens Al A   Mangan Michael W MW   Wain John J   Dorman Charles J CJ  

Science (New York, N.Y.) 20070101 5809


The Sfh protein is encoded by self-transmissible plasmids involved in human typhoid and is closely related to the global regulator H-NS. We have found that Sfh provides a stealth function that allows the plasmids to be transmitted to new bacterial hosts with minimal effects on their fitness. Introducing the plasmid without the sfh gene imposes a mild H-NS(-) phenotype and a severe loss of fitness due to titration of the cellular pool of H-NS by the A+T-rich plasmid. This stealth strategy seems t  ...[more]

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