Transcriptomics

Dataset Information

3

Transcription profiling of T. cruzi infection : 24 hours


ABSTRACT: Host cell infection by the intracellular pathogen, Trypanosoma cruzi, involves activation of signaling pathways, cytoskeletal reorganization, and targeted recruitment of host cell lysosomes. To determine the consequences of T. cruzi invasion on host cell gene expression, high density microarrays consisting of ~27,000 human cDNAs were hybridized with fluorescent probes generated from T. cruzi-infected human fibroblasts (HFF) at early time points following infection (2-24 h). Surprisingly, no genes were induced 2-fold in HFF between 2 and 6 h post-infection (hpi) in repeated experiments while immediate repression of six host cell transcripts was observed. A significant increase in transcript abundance for 106 host cell genes was observed at 24 hpi. Among the most highly induced is a set of interferon-stimulated genes, indicative of a type I interferon (IFN) response to T. cruzi. In support of this, T. cruzi-infected fibroblasts begin to secrete IFN at 18 hpi following the induction of IFN transcripts. As compared with global transcriptional responses evoked by other intracellular pathogens, T. cruzi is a stealth parasite that elicits few changes in host cell transcription during the initiation of infection.

ORGANISM(S): Homo sapiens  

SUBMITTER: Janos Demeter   Barbara Burleigh  

PROVIDER: E-SMDB-1560 | ArrayExpress | 2005-09-26

REPOSITORIES: ArrayExpress

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Publications

Immediate/early response to Trypanosoma cruzi infection involves minimal modulation of host cell transcription.

Vaena de Avalos Silvia S   Blader Ira J IJ   Fisher Michael M   Boothroyd John C JC   Burleigh Barbara A BA  

The Journal of biological chemistry 20011019 1


Host cell infection by the intracellular pathogen, Trypanosoma cruzi, involves activation of signaling pathways, cytoskeletal reorganization, and targeted recruitment of host cell lysosomes. To determine the consequences of T. cruzi invasion on host cell gene expression, high density microarrays consisting of approximately 27,000 human cDNAs were hybridized with fluorescent probes generated from T. cruzi-infected human fibroblasts (HFF) at early time points following infection (2-24 h). Surprisi  ...[more]

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