Transcriptomics

Dataset Information

4

Transcription profiling to analyse the effect of the date of preparation of human polyA RNA


ABSTRACT: This experiment was designed to assess the reproducibility of microarray hybridization using poly(A)+RNA. BC2 total RNA was isolated from which poly(A)+RNA was subsequently isolated. Three arrays were hybridized on the same day using arrays from the same batch. Two were done three months later using poly(A)+RNA freshly isolated from the same total RNA.

ORGANISM(S): Homo sapiens  

SUBMITTER: Janos Demeter   Hongjuan Zhao  

PROVIDER: E-SMDB-1613 | ArrayExpress | 2006-06-06

REPOSITORIES: ArrayExpress

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Publications

Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis.

Zhao Hongjuan H   Hastie Trevor T   Whitfield Michael L ML   Børresen-Dale Anne-Lise AL   Jeffrey Stefanie S SS  

BMC genomics 20021030 1


BACKGROUND:T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinomas and high-density cDNA microarrays. RESULTS:Using an optimized protocol, the average correlation coefficient of gene expression of 11,123 cDNA clones between amplified and unamplified samples is 0.82  ...[more]

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