Dataset Information


Transcription profiling of 3 polysome preparations from BY4741 yeast to identify translationally controlled genes

ABSTRACT: Red channel: 3 independent polysome preparations from BY4741 cells, grown in YPD to mid-log phase (OD 0.6-0.9). In each experiment 14 fractions were collected. In-vitro transcribed B.subtilis controls were added to each fraction and used for normalization. Green channel: Total RNA prepared from S288c cells.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Janos Demeter   Yoav Arava  

PROVIDER: E-SMDB-1883 | ArrayExpress | 2005-10-07


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Genome-wide analysis of mRNA translation profiles in Saccharomyces cerevisiae.

Arava Yoav Y   Wang Yulei Y   Storey John D JD   Liu Chih Long CL   Brown Patrick O PO   Herschlag Daniel D  

Proceedings of the National Academy of Sciences of the United States of America 20030326 7

We have analyzed the translational status of each mRNA in rapidly growing Saccharomyces cerevisiae. mRNAs were separated by velocity sedimentation on a sucrose gradient, and 14 fractions across the gradient were analyzed by quantitative microarray analysis, providing a profile of ribosome association with mRNAs for thousands of genes. For most genes, the majority of mRNA molecules were associated with ribosomes and presumably engaged in translation. This systematic approach enabled us to recogni  ...[more]

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