Dataset Information


Genome wide demarcation of RNA Polymerase II transcription Uuits Rrevealed by physical fractionation of chromatin- neutral enrichment

ABSTRACT: Genome-wide Demarcation of RNA Polymerase II Transcription Units Revealed by Physical Fractionation of Chromatin- Neutral enrichment

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jason Lieb   Janos Demeter  

PROVIDER: E-SMDB-2062 | ArrayExpress | 2005-10-07


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Genomewide demarcation of RNA polymerase II transcription units revealed by physical fractionation of chromatin.

Nagy Peter L PL   Cleary Michael L ML   Brown Patrick O PO   Lieb Jason D JD  

Proceedings of the National Academy of Sciences of the United States of America 20030515 11

Epigenetic modifications of chromatin serve an important role in regulating the expression and accessibility of genomic DNA. We report here a genomewide approach for fractionating yeast chromatin into two functionally distinct parts, one containing RNA polymerase II transcribed sequences, and the other comprising noncoding sequences and genes transcribed by RNA polymerases I and III. Noncoding regions could be further fractionated into promoters and segments lacking promoters. The observed separ  ...[more]

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