Transcriptomics

Dataset Information

32

Transcription profiling of human breast carcinoma tissues representing 930 patients with 17.4 years mean follow-up, basal cytokeratin expression was associated with decreased disease-specific survival


ABSTRACT: Expression profiling studies have classified breast carcinomas into luminal, normal breast-like, HER2 expressing and basal-like groups, with the latter two associated with poor outcomes. This study's objectives were to define an immunohistochemical profile that identifies basal-like tumors, to identify the presence of potential drug targets, and to determine the prognostic significance of the basal-like immunophenotype in a large series with long-term follow-up. On a panel of 21 breast tumors with basal-like expression profiles, we determined that this subtype was immunohistochemically negative for estrogen receptor and HER2, but positive for basal cytokeratins, HER1 and/or c-KIT. Using breast carcinoma tissue microarrays representing 930 patients with 17.4 years mean follow-up, basal cytokeratin expression was associated with decreased disease-specific survival. HER1 expression was observed in 54% of cases positive for basal cytokeratins (vs. 11% of negative cases) and was associated with poor survival independent of nodal status and size. c-KIT expression was more common in basal-like tumors than in other breast cancers, but did not influence prognosis. A panel of four antibodies (ER, HER1, HER2, and cytokeratin 5/6) can accurately identify basal-like tumors and suggests candidate drugs for therapies targeting HER1 or c-KIT.

ORGANISM(S): Homo sapiens  

SUBMITTER: Charles Perou  Janos Demeter  Therese Sorlie       

PROVIDER: E-SMDB-2863 | ArrayExpress| 2014-06-04

REPOSITORIES: ArrayExpress

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Publications

Immunohistochemical and clinical characterization of the basal-like subtype of invasive breast carcinoma.

Nielsen Torsten O TO   Hsu Forrest D FD   Jensen Kristin K   Cheang Maggie M   Karaca Gamze G   Hu Zhiyuan Z   Hernandez-Boussard Tina T   Livasy Chad C   Cowan Dave D   Dressler Lynn L   Akslen Lars A LA   Ragaz Joseph J   Gown Allen M AM   Gilks C Blake CB   van de Rijn Matt M   Perou Charles M CM  

Clinical cancer research : an official journal of the American Association for Cancer Research 20040801 16


PURPOSE: Expression profiling studies classified breast carcinomas into estrogen receptor (ER)+/luminal, normal breast-like, HER2 overexpressing, and basal-like groups, with the latter two associated with poor outcomes. Currently, there exist clinical assays that identify ER+/luminal and HER2-overexpressing tumors, and we sought to develop a clinical assay for breast basal-like tumors. EXPERIMENTAL DESIGN: To identify an immunohistochemical profile for breast basal-like tumors, we collected a se  ...[more]

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