Transcriptomics

Dataset Information

54

Chromatin immunoprecipitation of C. elegans intestine


ABSTRACT: For each experiment (as described in Pauli et al. 2006): Channel 1: whole worm lysate from strain SD1084 carrying transgene Pges-1::FLAG::PAB-1 Channel 2: mRNA immunoprecipitated from strain SD1084 carrying transgene Pges-1::FLAG::PAB-1 mRNA was amplified and hybridized to C. elegans DNA arrays from the Stuart Kim lab

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Min Jiang   Janos Demeter  

PROVIDER: E-SMDB-3592 | ArrayExpress | 2006-01-04

REPOSITORIES: ArrayExpress

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Publications

Chromosomal clustering and GATA transcriptional regulation of intestine-expressed genes in C. elegans.

Pauli Florencia F   Liu Yueyi Y   Kim Yoona A YA   Chen Pei-Jiun PJ   Kim Stuart K SK  

Development (Cambridge, England) 20051214 2


We used mRNA tagging to identify genes expressed in the intestine of C. elegans. Animals expressing an epitope-tagged protein that binds the poly-A tail of mRNAs (FLAG::PAB-1) from an intestine-specific promoter (ges-1) were used to immunoprecipitate FLAG::PAB-1/mRNA complexes from the intestine. A total of 1938 intestine-expressed genes (P<0.001) were identified using DNA microarrays. First, we compared the intestine-expressed genes with those expressed in the muscle and germline, and identifie  ...[more]

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