Transcriptomics

Dataset Information

5

Transcription profiling of human androgen receptor expressing prostate carcinoma cell line LNCaP and normal human foreskin fibroblasts expressing the androgen receptor treated with dihydrotestosterone (DHT), ethanol, or untreated vs. a common reference, see E-SMDB-3637


ABSTRACT: LNCaP cells are an established androgen receptor expressing prostate carcinoma cell line. Human foreskin fibroblasts also expressing the androgen receptor were obtained from phenotypic normal male individuals. Cells were cultured either at confluency leading to G0 cell cycle state or while they were proliferating. Cells were either untreated, or treated with dihydrotestosterone (DHT) or ethanol (ETOH) which also served as the solvent for the DHT. All experimental RNA samples derived from the untreated or treated cell lines were hybridized on cDNA arrays against a common reference. This reference was composed out of common reference CRG (50%) and out of fibroblast RNA (50%). This reference is also called "mixed reference" in the description of the 26 individual experiments.

ORGANISM(S): Homo sapiens  

SUBMITTER: Janos Demeter   Paul-Martin Holterhus  

PROVIDER: E-SMDB-3636 | ArrayExpress | 2006-11-06

REPOSITORIES: ArrayExpress

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Publications

Cell-line and tissue-specific signatures of androgen receptor-coregulator transcription.

Bebermeier Jan-Hendrik JH   Brooks James D JD   DePrimo Samuel E SE   Werner Ralf R   Deppe Uta U   Demeter Janos J   Hiort Olaf O   Holterhus Paul-Martin PM  

Journal of molecular medicine (Berlin, Germany) 20060824 11


Normal genital skin fibroblasts (GSF) and the human prostate carcinoma cell line LNCaP have been used widely as cell culture models of genital origin to study androgen receptor (AR) signaling. We demonstrate that LNCaP shows a reproducible response to androgens as assessed using cDNA-microarrays representing approximately 32,000 unique human genes, whereas several independent GSF strains are virtually unresponsive. We show that LNCaP cells express markedly higher AR protein levels likely contrib  ...[more]

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