Genomics

Dataset Information

4

Transcription profiling of Vibrio cholerae wild type, hapR and rpoS deletions in mid-exponential or stationary phase concluding that rpoS controls the Vibrio cholerae mucosal escape response


ABSTRACT: For expression analysis of wild-type V. cholerae, hapR, and rpoS deletion mutants in mid-exponential or stationary phase, the strains were grown to either OD600 of 0.3 or for 11 h in LB media at 37 0C, and bacteria from 2-ml culture were quickly pelleted, resuspended in Trizol reagent (GIBCO/BRL, San Diego, California, United States), and frozen on dry ice. RNA was isolated from the Trizol agent, treated with DNaseI (Ambion, Austin, Texas, United States), and cleaned by using the RNeasy kit (Qiagen, Valencia, California, United States). Labeling of cDNA and microarray hybridizations were performed as described [Yiliz et al. 2001, Mol. Micro. 53: 497-515]. Microarrays were scanned with a GenePix 400A instrument (Axon Instruments), using the GENEPIX 5.0 software. At least four microarray experiments were performed for each of two biological replicates for the tested strains. Gene expression of V. cholerae, rpoS, and hapR deletion mutants in stationary phase LB cultures was analyzed and compared to the wild-type parent under identical conditions. Gene expression of the wild-type parent during stationary phase after 11 h growth in LB was analyzed using RNA from an exponentially growing culture as a reference.

ORGANISM(S): Vibrio cholerae  

SUBMITTER: Janos Demeter   Nadia Dolganov   Alex Nielsen  

PROVIDER: E-SMDB-3833 | ArrayExpress | 2006-11-06

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-SMDB-3833.README.txt Txt
E-SMDB-3833.idf.txt Idf
E-SMDB-3833.processed.1.zip Processed
E-SMDB-3833.raw.1.zip Raw
E-SMDB-3833.sdrf.txt Txt
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Publications

RpoS controls the Vibrio cholerae mucosal escape response.

Nielsen Alex Toftgaard AT   Dolganov Nadia A NA   Otto Glen G   Miller Michael C MC   Wu Cheng Yen CY   Schoolnik Gary K GK  

PLoS pathogens 20061001 10


Vibrio cholerae causes a severe diarrhoeal disease by secreting a toxin during colonization of the epithelium in the small intestine. Whereas the initial steps of the infectious process have been intensively studied, the last phases have received little attention. Confocal microscopy of V. cholerae O1-infected rabbit ileal loops captured a distinctive stage in the infectious process: 12 h post-inoculation, bacteria detach from the epithelial surface and move into the fluid-filled lumen. Designat  ...[more]

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