Transcriptomics

Dataset Information

26

Transcription profiling of mouse embryonic stem cell line D3 differentiation cultures treated by different concentrations of flusilazole.


ABSTRACT: This study investigated the effect of the compound concentration of Flusilazole on gene expression responses in Embryonic Stem Cell Differentiation Cultures. Flusilazole is used in agriculture as a fungicide, but has developmental toxic effects. To study the effects of its concentration on development, we exposed Embryonic Stem Cell Differentiation Cultures to six concentrations and a control. Gene expression responses after 24h exposure were compared to untreated samples at 0, 24 and 48 h using microarray gene expression profiling.

ORGANISM(S): Mus musculus  

SUBMITTER: Jeroen Pennings  

PROVIDER: E-TABM-1027 | ArrayExpress | 2011-07-02

REPOSITORIES: ArrayExpress

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Publications

Complementary detection of embryotoxic properties of substances in the neural and cardiac embryonic stem cell tests.

Theunissen Peter T PT   Pennings Jeroen L A JL   van Dartel Dorien A M DA   Robinson Joshua F JF   Kleinjans Jos C S JC   Piersma Aldert H AH  

Toxicological sciences : an official journal of the Society of Toxicology 20121213 1


In developmental toxicity testing, in vitro screening assays are highly needed to increase efficiency and to reduce animal use. A promising in vitro assay is the cardiac embryonic stem cell test (ESTc), in which the effect of developmental toxicants on cardiomyocyte differentiation is assessed. Recently, we developed a neural differentiation variant of the stem cell test (neural embryonic stem cell test [ESTn]). In both of these models, we have previously performed a series of transcriptomic stu  ...[more]

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