Transcriptomics

Dataset Information

481

ChIP-chip by array of yeast to determine genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms, and transcription factors


ABSTRACT: To investigate the chromatin transcription cycle, we determined genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms, and transcription factors in growing yeast. ChIP-chip was performed to identify the genomic binding locations for Rpb3, TFIIB, Tfg1, Kin28, Cet1, Spt4, Spt5, Spt6, Elf1, Spn1, Bur1, Ctk1, Paf1, Spt16, Pcf11, and Rpb1 phosphorylated at serine 2, 5, and 7 residues of the CTD, respectively.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Michael Lidschreiber  

PROVIDER: E-TABM-1033 | ArrayExpress | 2010-08-23

REPOSITORIES: ArrayExpress

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Publications

Uniform transitions of the general RNA polymerase II transcription complex.

Mayer Andreas A   Lidschreiber Michael M   Siebert Matthias M   Leike Kristin K   Söding Johannes J   Cramer Patrick P  

Nature structural & molecular biology 20100905 10


We present genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms and transcription factors in proliferating yeast. Pol II exchanges initiation factors for elongation factors during a 5' transition that is completed 150 nucleotides downstream of the transcription start site (TSS). The resulting elongation complex is composed of all the elongation factors and shows high levels of Ser7 and Ser5 phosphorylation on the C-terminal repeat domain (CTD) of Pol II. Ser2 phos  ...[more]

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