Dataset Information


Transcription profiling by array of Plasmodium berghei wild type and puf2 knock out in a time series

ABSTRACT: Many eukaryotic developmental and cell fate decisions are effected post-transcriptionally that mechanistically involve RNA binding proteins as regulators of translation of key mRNAs. In the unicellular eukaryote malaria parasite, Plasmodium, one of the most dramatic changes in cell morphology and function occurs during transmission between mosquito and human host. In the mosquito salivary glands, Plasmodium sporozoites are slender, motile and remain infectious for several weeks; only after transmission and liver cell invasion, does the parasite rapidly transform into a round, non-motile exo-erythrocytic form (EEF) that gives rise to thousands of infectious merozoites to be released into the blood stream. Here we demonstrate a Plasmodium homolog of the RNA binding protein, Pumilio, as a key regulator of the sporozoite to EEF transformation. In the absence of Pumilio-2 (Puf2) Plasmodium berghei sporozoites initiate early stage EEF development inside mosquito salivary glands with characteristic morphological changes; puf2- salivary gland sporozoites lose gliding motility, cell traversal ability and are less infective. Global expression profiling confirmed that transgenic parasites exhibit genome-wide transcriptional adaptations typical for Plasmodium intra-hepatic development. The data demonstrate that Puf2 is a key player in regulating developmental control, and imply that transformation of salivary gland-resident sporozoites into early liver stage parasites is regulated by a post-translational mechanism.

ORGANISM(S): Plasmodium berghei  

SUBMITTER: Celine Carret  

PROVIDER: E-TABM-1067 | ArrayExpress | 2010-11-01


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Transition of Plasmodium sporozoites into liver stage-like forms is regulated by the RNA binding protein Pumilio.

Gomes-Santos Carina S S CS   Braks Joanna J   Prudêncio Miguel M   Carret Céline C   Gomes Ana Rita AR   Pain Arnab A   Feltwell Theresa T   Khan Shahid S   Waters Andrew A   Janse Chris C   Mair Gunnar R GR   Mota Maria M MM  

PLoS pathogens 20110519 5

Many eukaryotic developmental and cell fate decisions that are effected post-transcriptionally involve RNA binding proteins as regulators of translation of key mRNAs. In malaria parasites (Plasmodium spp.), the development of round, non-motile and replicating exo-erythrocytic liver stage forms from slender, motile and cell-cycle arrested sporozoites is believed to depend on environmental changes experienced during the transmission of the parasite from the mosquito vector to the vertebrate host.  ...[more]

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