Transcriptomics

Dataset Information

5

ChIP-chip mapping of RNA polymerase II binding in D.melanogaster stage 5 embryos


ABSTRACT: ChIP-chip was performed using chromatin isolated from formaldehyde crosslinked, stage 5 embryos, and 8WG16 monoclonal antibody against RNA polymerase II(Covance Inc.). The chromatin immunoprecitation and hybridization for each antibody were carried out in duplicates, and in each chromatin immunoprecitation and hybridization series, two mock IP controls and two input DNA controls were also generated. The microarray used in this study is the Affymetrix Drosophila genomic DNA tiling array, which has about 3 million oligo pairs (perfect match-mismatch), covering the whole euchromatic sequences of the fly genome at a resolution of about 35 bp.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Michael Eisen  

PROVIDER: E-TABM-1130 | ArrayExpress | 2011-02-28

REPOSITORIES: ArrayExpress

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