Project description:Roswell Park Human 19K Array CGH<br><br>the format of the data is:<br> <br>Reporter Identifier<br>Band<br>Count - number of replicate spots the data represents (some spots are excluded in image analysis, usually because they are too dim to be reliable)<br>chr - Chromosome<br>Start - Start location in base pairs<br>Stop - Stop Location in Base pairs<br>Center - Center in base pairs<br>g_loc - graphing location this is the location of the center in base pairs if the chromosomes were laid end to end<br>Log2 ratio - log2 ratio (test/control)<br>Ratio - linear version of the ratio above <br>A - Measure of brightness A=(log2 cy3 + log2 cy5)/2 <br>Flag - Used to color code spots<br> 3 - red probably mismapped<br> 4 - green potentially polymorphic<br> 5 - light blue Shows a high degree of duplication with other area in the genome (see UCSC genome browser) <br>reference - Reference for why the clone was flagged <br>Pub Med link - Pub Med ID for why clone was flagged
Project description:Extensive gene methylation correlated strongly with transcriptional silencing and differential expression under specific conditions. DNA methylation and its role in gene regulation is conserved in stramenopile. Methylome of the whole genome of diatom phaeodactylum tricornutum.
Project description:Chromatin immunoprecipitation and hybridization to a chromosome-wide DNA tiling array (ChIP-chip)was performed to compare the distribution pattern of H3K9me2 between nrpd1a-4_nrpd1b-11 and wild type. Experiments were done using two independent biological replicates.<br>
Project description:The aim was to study the effects of Nur77 on the white adipose tissue transcriptome after fasting. For this purpose we performed gene expression profiling of white adipose tissue from Nur77-/- mice and wildtype (Nur77+/+) littermates submitted to prolonged fasting using microarray analysis on >27k elements cDNA microarrays.