Dataset Information


Poly(A) tail length profiling of mRNAs from fission yeast

ABSTRACT: To obtain global data on polyadenylation of mRNAs, we fractionated the mRNAs according to their poly(A) tail length using a poly-U sepharose column followed by differential elution at five temperatures. Five mRNA fractions with distinct ranges of poly(A) tail length were then hybridized to microarrays using total eluate as a reference.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Daniel Lackner  

PROVIDER: E-TABM-217 | ArrayExpress | 2007-05-16


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A network of multiple regulatory layers shapes gene expression in fission yeast.

Lackner Daniel H DH   Beilharz Traude H TH   Marguerat Samuel S   Mata Juan J   Watt Stephen S   Schubert Falk F   Preiss Thomas T   Bähler Jürg J  

Molecular cell 20070401 1

Gene expression is controlled at multiple layers, and cells may integrate different regulatory steps for coherent production of proper protein levels. We applied various microarray-based approaches to determine key gene-expression intermediates in exponentially growing fission yeast, providing genome-wide data for translational profiles, mRNA steady-state levels, polyadenylation profiles, start-codon sequence context, mRNA half-lives, and RNA polymerase II occupancy. We uncovered widespread and  ...[more]

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