Transcriptomics

Dataset Information

52

Transcription profiling of human breast cancer cell lines and tissue samples to identify aberrant microRNA expression


ABSTRACT: MicroRNAs are a class of small non-coding RNAs that control gene expression by targeting messenger RNAs and triggering either translation repression or RNA degradation. Their aberrant expression may be involved in human diseases, including cancer. Indeed, microRNA aberrant expression has been previously found in human chronic lymphocytic leukemias, where microRNA signatures were associated with specific clinico-biological features. Here, we show that, in comparison to normal breast tissue, microRNAs are also aberrantly expressed in human breast cancer. The overall microRNA expression could clearly separate normal versus cancer tissues, with the most significantly deregulated microRNAs being mir-125b, mir-145, mir-21, mir-155. Results were confirmed by microarray and Northern blot analyses. We could identify microRNAs whose expression was correlated with specific breast cancer bio-pathologic features, such as estrogen and progesterone receptor expression, tumor stage, vascular invasion or proliferation index.

INSTRUMENT(S): ScanArray 4000XL [PerkinElmer]

ORGANISM(S): Homo sapiens  

DISEASE(S): Normal,Breast Cancer

SUBMITTER: Eros Magri  Italo Nenci  Angelo Veronese  Carlo M. Croce  Manuela Campiglio  Muller Fabbri  Riccardo Spizzo  Sylvie Menard  Manuela Ferracin  Massimo Negrini  Anne Rosenberg  Massimo Pedriali  George A. Calin  Silvia Sabbioni  Marilena V. Iorio  Juan P. Palazzo  Chang-Gong Liu  Stefano Volinia  Patrizia Querzoli  Piero Musiani 

PROVIDER: E-TABM-23 | ArrayExpress | 2006-01-01

REPOSITORIES: ArrayExpress

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Publications


Small noncoding microRNAs (miRNAs) can contribute to cancer development and progression and are differentially expressed in normal tissues and cancers. From a large-scale miRnome analysis on 540 samples including lung, breast, stomach, prostate, colon, and pancreatic tumors, we identified a solid cancer miRNA signature composed by a large portion of overexpressed miRNAs. Among these miRNAs are some with well characterized cancer association, such as miR-17-5p, miR-20a, miR-21, miR-92, miR-106a,  ...[more]

Publication: 1/2

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