Transcriptomics

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Transcription profiling of human MCF7 estradiol treated cells sampled at 0h, 3h, 6h, 12h, 24h after treatment identifies an extensive ER-alpha binding program on human gene promoters


ABSTRACT: Gene expression experiments using the Illumina Human Ref8 whole genome expression array system. MCF7 cells were treated with estradiol and then used for expression experiments at specific timepoints after treatment (0h, 3h, 6h, 12h, 24h).

ORGANISM(S): Homo sapiens  

SUBMITTER: Kasey Hutt   

PROVIDER: E-TABM-231 | ArrayExpress | 2012-02-23

REPOSITORIES: ArrayExpress

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Publications

Sensitive ChIP-DSL technology reveals an extensive estrogen receptor alpha-binding program on human gene promoters.

Kwon Young-Soo YS   Garcia-Bassets Ivan I   Hutt Kasey R KR   Cheng Christine S CS   Jin Mingjie M   Liu Dongyan D   Benner Chris C   Wang Dong D   Ye Zhen Z   Bibikova Marina M   Fan Jian-Bing JB   Duan Lingxun L   Glass Christopher K CK   Rosenfeld Michael G MG   Fu Xiang-Dong XD  

Proceedings of the National Academy of Sciences of the United States of America 20070314 12


ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust analysis with much reduced materials compared with standard procedures. We profiled general and sequence-specific DNA binding transcription fa  ...[more]

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