Metabolic Syndrome (MetS) is highly prevalent and has considerable public health impact, but its underlying genetic factors remain elusive. To identify gene networks involved in MetS, we conducted whole-genome expression and genotype profiling on abdominal (ABD) and gluteal (GLU) adipose tissue, and whole blood (WB), from 29 MetS cases and 44 controls. Co-expression network analysis for each tissue independently identified nine, six, and zero MetS-associated modules of coexpressed genes in ABD, ...[more]
Project description:The genomes of Bordetella pertussis strains with different passage histories were digested with a restriction endonuclease and separated by pulsed-field gel electrophoresis (PFGE). Each PFGE fragment was labeled and hybridized to a microarray to assess the genome content of the fragment.
Project description:B. pertussis GMT-1 was grown in media containing magnesium sulfate to put the culture in the Bvg- phase. The cells were then washed and grown in media lacking magnesium sulfate and samples were harvested at time=0 and several time points beyond.