Transcriptomics

Dataset Information

6

Translation profiling of fission yeast wild type and rng3-65 fission yeast to identify changes in th enumber of ribosomes associated with specific transcripts


ABSTRACT: Genome-wide translational profiling of rng3-65 compared to wild type cells. We used sucrose gradients to separate RNAs according to the number of associated ribosomes (a surrogate for translational efficiency). Preparation of the extracts and fractionation was carried out as described in Lackner et al, 2007 (Mol Cell 26(1):145-55). The fractions were pooled into four groups (1 closest to the top, i.e. not associated with ribosomes and 4 closest to the bottom, i.e., associated with polysomes). RNA was extracted from the pools and the corresponding pools from wild type and mutant cells were directly compared using DNA microarrays. Changes in translation are expected to alter the number of ribosomes associated with specific transcripts, and therefore result in a redistribution of the RNAs across the different fractions.

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Juan Mata  

PROVIDER: E-TABM-565 | ArrayExpress | 2008-12-23

REPOSITORIES: ArrayExpress

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Publications

Rng3, a member of the UCS family of myosin co-chaperones, associates with myosin heavy chains cotranslationally.

Amorim Maria J MJ   Mata Juan J  

EMBO reports 20081219 2


The production of functional myosin heavy chains in many eukaryotic organisms requires the function of proteins containing UCS domains (UNC-45/CRO1/She4), which bind to the myosin head domain and stimulate its folding. UCS proteins are essential for myosin-related functions such as muscle formation, RNA localization and cytokinesis. Here, we show that the Schizosaccharomyces pombe UCS protein Rng3 associates with polysomes, suggesting that UCS proteins might assist myosin folding cotranslational  ...[more]

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