Transcriptomics

Dataset Information

7

Transcription profiling of mouse spleen CD4+ CD44Low CD62LHigh cells stimulated with anti-CD3 or anti-CD28 in the presence or absence of hTGFb-1 and mouse IL-6


ABSTRACT: Mouse spleen CD4+CD44LowCD62LHigh cells were stimulated with 2ug/ml anti-CD3(eBioscience), 2ug/ml anti-CD28 (eBioscience) in the presence (Th17 condition) or absence (Th0 condition) of hTGFb-1 (1ng/ml eBioscience), mouse IL-6(10ng/ml eBioscience). Cells were collected after 24 hourÕs culture using RNAeasy columns (Qiagen) for total RNA isolation. Isolated total RNA from Th0 and Th17 conditioned cells were submitted for the microarray gene transcription comparison analysis using Affymetrix Mouse 430A 2.0 array chips.

ORGANISM(S): Mus musculus  

SUBMITTER: Hulin Jin and.Vijay.K. Kuchroo  

PROVIDER: E-TABM-583 | ArrayExpress | 2008-12-21

REPOSITORIES: ArrayExpress

Dataset's files

Source:
Action DRS
E-TABM-583.README.txt Txt
E-TABM-583.idf.txt Idf
E-TABM-583.processed.1.zip Processed
E-TABM-583.sdrf.txt Txt
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