Transcription profiling of fission yeast wild type and yox1 mutant strains
ABSTRACT: Transcription profiling of fission yeast /yox1/ deletion and genome wide location analysis of Yox1p and Cdc10p transcription factors reveal a negative feedback interaction: /yox1 /is transcriptionally activated by MBF, and Yox1p in turn transcriptionally represses the MBF target genes (including /yox1 /itself).
The regulation of the G1- to S-phase transition is critical for cell-cycle progression. This transition is driven by a transient transcriptional wave regulated by transcription factor complexes termed MBF/SBF in yeast and E2F-DP in mammals. Here we apply genomic, genetic, and biochemical approaches to show that the Yox1p homeodomain protein of fission yeast plays a critical role in confining MBF-dependent transcription to the G1/S transition of the cell cycle. The yox1 gene is an MBF target, and ...[more]
Project description:Recent studies in comparative genomics demonstrate that inter-species comparisons represent a powerful tool for revealing both conserved and specialized biological processes across large evolutionary distances. All cells need to adjust to environmental fluctuations in metal levels since both metal excess and deficit are detrimental. Here, we explore the conservation of metal homoeostasis in two distantly related yeasts, examining genome-wide gene expression responses to changing copper and iron levels in budding and fission yeast using DNA microarrays